In conclusion this study indicates that chronic CLA treatment inhibits DCA-induced PKC and NF-kappaB activation in colon cancer cells. Acute CLA treatment had no effect on PKC or NF-kappaB activation. Chronic c-9,t-11-CLA and t-10,c-12-CLA treatment inhibited DCA-induced PKC beta1 and PKC delta translocation and also inhibited NF-kappaB activation. PKC translocation was observed using real-time photomicroscopy and fluorescent microscopy and NF-kappaB analyses by gel shift assays. from a scorpion venom-structural heterogeneity induced by proline cis/trans isomerization. HCT116 cells were treated with 100 mumol/l and 50 mumol/l cis-9,trans-11-CLA and trans-10,cis-12-CLA for 24 h and 14 days, respectively. Notice sur lInstitut Pasteur dAlgrie, tome II, Alger. The aim of this study was to examine acute and chronic, isomer-specific effects of CLA on bile salt-induced PKC and NF-kappaB signal transduction in human colon cancer cells.
Secondary bile acids on the other hand are known as tumour promoters in colon cancer with effects on protein kinase C (PKC) and nuclear factor kappa B (NF-kappaB) signalling pathways. Korea, ASEAN, Germany, France, UK, Italy, Spain, CIS, and Brazil etc. Conjugated linoleic acid (CLA) has anti-carcinogenic effects in a variety of cancers including colon cancer.